6YLI

Crystal structure of human bcl-xL bound to trichoplax adhaerens trBak BH3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	39.141, 63.801, 69.761
Unit cell angles	90.00, 94.57, 90.00

Refinement procedure

Resolution	39.020 - 1.900
R-factor	0.2007
Rwork	0.199
R-free	0.23590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pq1
RMSD bond length	0.008
RMSD bond angle	0.914
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.070	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.125	1.737
Number of reflections	26735	1963
<I/σ(I)>	6.4
Completeness [%]	98.2	97.5
Redundancy	5.5
CC(1/2)	0.996	0.351

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.2 M ammonium sulfate and 30 % w/v PEG 8000