6YJO
Structure of FgChi7B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-02 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.600, 67.455, 85.082 |
| Unit cell angles | 90.00, 116.31, 90.00 |
Refinement procedure
| Resolution | 38.130 - 2.380 |
| R-factor | 0.2137 |
| Rwork | 0.212 |
| R-free | 0.23760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k8e |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.665 |
| Data reduction software | XDS (20180106) |
| Data scaling software | XSCALE (20180106) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.130 | 2.465 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.139 | 1.573 |
| Rpim | 0.055 | 0.621 |
| Number of reflections | 22534 | 2206 |
| <I/σ(I)> | 10.79 | 1.17 |
| Completeness [%] | 98.3 | 97.35 |
| Redundancy | 7.3 | 7.3 |
| CC(1/2) | 0.997 | 0.454 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | PEG 3350, 22% (w/v) Sodium potassium phosphate, 200 mM |
