6YJK

Structure of CYRI-B (FAM49B) from Rhincodon typus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	39.810, 72.230, 107.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.007 - 2.370
R-factor	0.2276
Rwork	0.225
R-free	0.27720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yjj
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.010	60.010	2.460
High resolution limit [Å]	2.370	8.870	2.370
Rmerge	0.127	0.045	1.030
Rmeas	0.134	0.048	1.107
Rpim	0.041	0.016	0.396
Total number of observations	138576	2561	10214
Number of reflections	13266	313	1362
<I/σ(I)>	10.1	28.5	2
Completeness [%]	100.0	99.9	100
Redundancy	10.4	8.2	7.5
CC(1/2)	0.998	0.998	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288.15	30% (w/v) glycerol ethoxylate, 0.2 M ammonium acetate and 0.1 M MES pH 6.5