6YJG
Crystal structure of MINDY1 mutant-Y114F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-02 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.87313 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 102.160, 102.160, 162.714 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.210 - 3.280 |
| R-factor | 0.2071 |
| Rwork | 0.205 |
| R-free | 0.24620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jkn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.040 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 72.240 | 72.240 | 3.540 |
| High resolution limit [Å] | 3.280 | 8.670 | 3.280 |
| Rmerge | 0.282 | 0.049 | 1.765 |
| Rmeas | 0.288 | 0.050 | 1.799 |
| Rpim | 0.055 | 0.010 | 0.343 |
| Total number of observations | 378950 | 19774 | 74980 |
| Number of reflections | 13867 | 865 | 2781 |
| <I/σ(I)> | 11.5 | 38.1 | 2.5 |
| Completeness [%] | 100.0 | 99.9 | 99.8 |
| Redundancy | 27.3 | 22.9 | 27 |
| CC(1/2) | 0.998 | 1.000 | 0.902 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1 M Tris-HCl pH 8.5, 1.5 M Ammonium phosphate dibasic |
