6YGU
Crystal structure of the minimal Mtr4-Red1 complex (single chain) from Chaetomium thermophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.2741 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.979, 88.906, 168.367 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.458 - 1.990 |
| R-factor | 0.2068 |
| Rwork | 0.204 |
| R-free | 0.25160 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.317 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.460 | 2.061 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.103 | 1.332 |
| Number of reflections | 47338 | 4660 |
| <I/σ(I)> | 13.45 | 1.55 |
| Completeness [%] | 99.9 | 99.79 |
| Redundancy | 13 | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291.15 | 0.2 M Magnesium Acetate 20% PEG 3350 |
