6YFW
TGT H333F mutant crystallised at pH 8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.596, 64.971, 70.507 |
| Unit cell angles | 90.00, 95.92, 90.00 |
Refinement procedure
| Resolution | 43.410 - 1.260 |
| R-factor | 0.1313 |
| Rwork | 0.130 |
| R-free | 0.15410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pud |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.925 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.410 | 1.340 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Number of reflections | 106813 | 17052 |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 98.0 | |
| Redundancy | 3 | |
| CC(1/2) | 0.998 | 0.809 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 288 | 100 mM TRIS pH 8.5, 1 mM DTT, 10% (v/v) DMSO, 13% (m/v) PEG8000 |
