Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 469.425, 332.407, 293.528 |
| Unit cell angles | 90.00, 127.87, 90.00 |
Refinement procedure
| Resolution | 57.200 - 3.100 |
| R-factor | 0.2501 |
| Rwork | 0.250 |
| R-free | 0.25370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | FFT |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 57.200 | 57.200 | 3.270 |
| High resolution limit [Å] | 3.100 | 9.800 | 3.100 |
| Rmerge | 0.061 | 1.139 | |
| Rmeas | 0.368 | 0.070 | 1.462 |
| Rpim | 0.193 | 0.033 | 0.901 |
| Total number of observations | 1699944 | 81249 | 65046 |
| Number of reflections | 508294 | 19979 | 33270 |
| <I/σ(I)> | 3.4 | 9.3 | 0.7 |
| Completeness [%] | 79.5 | 97.5 | 35.7 |
| Redundancy | 3.3 | 4.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.615 M sodium succinate pH 7.0 |
