Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 415.014, 334.996, 291.781 |
| Unit cell angles | 90.00, 134.66, 90.00 |
Refinement procedure
| Resolution | 49.140 - 3.484 |
| R-factor | 0.2685 |
| Rwork | 0.269 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | FFT |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.168 | 49.168 | 3.690 |
| High resolution limit [Å] | 3.480 | 10.250 | 3.480 |
| Rmerge | 0.449 | 0.059 | 2.461 |
| Rmeas | 0.531 | 0.070 | 2.903 |
| Total number of observations | 1226578 | ||
| Number of reflections | 353695 | 13916 | 54664 |
| <I/σ(I)> | 3.18 | 18.09 | 0.48 |
| Completeness [%] | 97.9 | 96 | 94.2 |
| Redundancy | 3.468 | 3.274 | 3.464 |
| CC(1/2) | 0.966 | 0.995 | 0.180 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 12.5 % PEG 3350, 0.05 M Bis-tris pH 5.5 |
