Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-09 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 282.018, 302.810, 352.159 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.079 - 2.762 |
| R-factor | 0.2446 |
| Rwork | 0.244 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | FFT |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.079 | 60.079 | 2.910 |
| High resolution limit [Å] | 2.762 | 8.730 | 2.762 |
| Rmerge | 0.051 | 1.532 | |
| Rmeas | 0.401 | 0.057 | 1.692 |
| Rpim | 0.169 | 0.024 | 0.703 |
| Total number of observations | 2023525 | ||
| Number of reflections | 378707 | 12295 | 55322 |
| <I/σ(I)> | 4.7 | 10.7 | 0.5 |
| Completeness [%] | 99.4 | 98.5 | 99.8 |
| Redundancy | 5.3 | 5.3 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 5% PEG 3000, 0.08 M zinc acetate, 0.05 M sodium acetate pH 4.6 |
