Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | H 3 |
| Unit cell lengths | 278.203, 278.203, 661.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.003 - 3.700 |
| R-factor | 0.2352 |
| Rwork | 0.235 |
| R-free | 0.23750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | FFT |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.003 | 38.003 | 3.900 |
| High resolution limit [Å] | 3.700 | 11.700 | 3.700 |
| Rmerge | 0.091 | 0.817 | |
| Rmeas | 0.265 | 0.106 | 1.137 |
| Rpim | 0.146 | 0.052 | 0.788 |
| Total number of observations | 338161 | 23021 | 10449 |
| Number of reflections | 132293 | 6047 | 8520 |
| <I/σ(I)> | 3.2 | 9.7 | 0.6 |
| Completeness [%] | 64.9 | 93.8 | 28.5 |
| Redundancy | 2.6 | 3.8 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20 % PEG 10000, 0.1 M HEPES pH 7.5 |
