6YFI

Bacteriophage EMS014 coat protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	71.558, 92.699, 94.819
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.210 - 1.248
R-factor	0.1764
Rwork	0.175
R-free	0.19740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Poly(Ala) model of ESE021 CP dimer
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.615	48.615	1.320
High resolution limit [Å]	1.248	3.730	1.250
Rmerge	0.085	0.058	1.054
Rmeas	0.092	0.063	1.152
Number of reflections	173301	6916	26527
<I/σ(I)>	8.51	26.62	1.03
Completeness [%]	99.1	99.7	94.7
Redundancy	6.405	5.996	6.037
CC(1/2)	0.998	0.998	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	10% PEG 8000, 0.1 M sodium chloride, 0.05 M phosphate/citrate pH 4.2