6YF9

Virus-like particle of bacteriophage AVE002

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	278.090, 390.209, 554.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.205 - 3.196
R-factor	0.2611
Rwork	0.261
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ms2
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	FFT
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.205	62.205	3.370
High resolution limit [Å]	3.196	10.110	3.200
Rmerge	0.672	0.093	1.727
Rmeas	0.731	0.102	1.894
Rpim	0.285	0.040	0.764
Total number of observations		197738	812124
Number of reflections	970502	31514	138209
<I/σ(I)>	3.3	11.1	1.1
Completeness [%]	98.8	97.7	97
Redundancy	6.3	6.3	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	46% MPD, 0.1 M ammonium nitrate, 0.1 M MES pH 6.5