6YF6
EclA C-terminal domain; sugar-binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-24 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.512, 63.671, 134.009 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.570 - 2.000 |
| R-factor | 0.1923 |
| Rwork | 0.190 |
| R-free | 0.23710 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.606 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | Aimless (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.160 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 22990 | 1594 |
| <I/σ(I)> | 35.6 | |
| Completeness [%] | 99.6 | |
| Redundancy | 25.6 | |
| CC(1/2) | 1.000 | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 100-280 mM disodium phosphate, 16-22% (w/v) PEG 3350 |
