6YEX

VcaM4I restriction endonuclease in the absence of DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0064
Spacegroup name	P 21 21 21
Unit cell lengths	56.970, 93.532, 126.261
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.480 - 1.500
R-factor	0.1936
Rwork	0.193
R-free	0.21320
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.131
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.590
High resolution limit [Å]	1.500	1.500
Rmeas	0.046	1.104
Number of reflections	107966	17067
<I/σ(I)>	26.06	2.27
Completeness [%]	99.2	98.2
Redundancy	13.1	13.4
CC(1/2)	1.000	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	Protein (7.2 mg/ml) in 0.27 M NaCl, 13.5 mM Tris-HCl pH 8.5, 0.2 M sodium malonate and 0.9 mM TCEP was mixed in 1:1 ratio with reservoir solution (1.8 M ammonium sulfate, 0.1 M MES pH 5.25). Perfluoropolyether oil was used as a cryoprotectant.