6YES

Crystal structure of type I-D CRISPR-Cas nuclease Cas10d

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	141.430, 141.430, 223.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	91.310 - 4.100
R-factor	0.3446
Rwork	0.343
R-free	0.37030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6thh
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.310	4.700
High resolution limit [Å]	4.000	4.620
Rmerge		0.520
Number of reflections	23124	674
<I/σ(I)>	0.32
Completeness [%]	99.3	100
Redundancy	9.5
CC(1/2)	0.160	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	90 mM Hepes-NaOH 7 % (w/v) PEG8000 7 % (v/v) Ethylene glycol 1 % (w/v) PEG3350