6YEB

E.coli's Putrescine receptor PotF in its closed apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 32 2 1
Unit cell lengths	71.118, 71.118, 272.420
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.680 - 1.970
R-factor	0.1916
Rwork	0.191
R-free	0.21920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a99
RMSD bond length	0.009
RMSD bond angle	0.934
Data reduction software	XDS (20180126)
Data scaling software	XDS (20180126)
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.680	2.040
High resolution limit [Å]	1.970	1.970
Rmerge	0.124	3.324
Rmeas	0.130	3.480
Rpim	0.039	1.021
Number of reflections	58117	5691
<I/σ(I)>	12.16	0.68
Completeness [%]	100.0	99.93
Redundancy	10.9	11.3
CC(1/2)	0.999	0.230

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.8	293	2.4 M Ammoniumsulfate, 0.1 M Bicine pH 8.8, 5% PEG 3550