6YE7
E.coli's Putrescine receptor PotF complexed with Cadaverine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 70.869, 70.869, 271.185 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.500 - 1.600 |
| R-factor | 0.1684 |
| Rwork | 0.168 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a99 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.938 |
| Data reduction software | XDS (20180126) |
| Data scaling software | XDS (20180126) |
| Phasing software | PHASER (2.8.1) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.500 | 1.657 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.091 | 2.540 |
| Rmeas | 0.095 | 2.691 |
| Rpim | 0.029 | 0.866 |
| Number of reflections | 104898 | 9828 |
| <I/σ(I)> | 14.85 | 0.66 |
| Completeness [%] | 99.2 | 94.29 |
| Redundancy | 10.8 | 9 |
| CC(1/2) | 0.999 | 0.231 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 293 | 0.1 M Bicine pH 8.8, 2.4 M Ammoniumsulfate, 5% PEG 3550 |
