6YE6

E.coli's Putrescine receptor PotF complexed with Agmatine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 32 2 1
Unit cell lengths	71.161, 71.161, 272.866
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.730 - 1.560
R-factor	0.1716
Rwork	0.171
R-free	0.18520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a99
RMSD bond length	0.010
RMSD bond angle	1.000
Data reduction software	XDS (20180126)
Data scaling software	XDS (20180126)
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.17.1-3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.730	1.616
High resolution limit [Å]	1.560	1.560
Rmerge	0.065	2.922
Rmeas	0.068	3.064
Rpim	0.021	0.911
Number of reflections	115429	11384
<I/σ(I)>	18.64	0.69
Completeness [%]	99.8	99.85
Redundancy	11	11.1
CC(1/2)	1.000	0.272

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.8	293	2.4 M Ammoniumsulfate, 0.1 M Bicine pH 8.8, 7% Jeffamine M600