6YE0
E.coli's Putrescine receptor PotF complexed with Putrescine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 70.987, 70.987, 272.559 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.640 - 1.630 |
| R-factor | 0.1727 |
| Rwork | 0.172 |
| R-free | 0.20020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a99 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.908 |
| Data reduction software | XDS (20180409) |
| Data scaling software | XDS (20180409) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1-3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.640 | 1.688 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.122 | 2.482 |
| Rmeas | 0.128 | 2.618 |
| Rpim | 0.039 | 0.819 |
| Number of reflections | 100972 | 9934 |
| <I/σ(I)> | 10.07 | 0.69 |
| Completeness [%] | 99.9 | 99.81 |
| Redundancy | 10.7 | 9.9 |
| CC(1/2) | 0.998 | 0.274 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 293 | 2.4 M Ammoniumsulfate, 0.1 M Bicine pH 8.8, 4.5% Jeffamine M600 |
