6YDR
Crystal structure of Mengla Virus VP30 C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 73.062, 73.062, 53.459 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.870 - 1.340 |
| R-factor | 0.12848 |
| Rwork | 0.127 |
| R-free | 0.15496 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5t3w |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.325 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.870 | 1.392 |
| High resolution limit [Å] | 1.340 | 1.344 |
| Rmerge | 0.065 | 0.438 |
| Rmeas | 0.073 | 0.491 |
| Rpim | 0.030 | 0.216 |
| Number of reflections | 36367 | 3598 |
| <I/σ(I)> | 16.27 | 2.82 |
| Completeness [%] | 99.9 | |
| Redundancy | 5.4 | |
| CC(1/2) | 0.993 | 0.852 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Ammonium Sulfate , 0.1M Sodium, Acetate pH4.6, 25% PEG4000 |
