6YDR

Crystal structure of Mengla Virus VP30 C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 32 1 2
Unit cell lengths	73.062, 73.062, 53.459
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.870 - 1.340
R-factor	0.12848
Rwork	0.127
R-free	0.15496
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t3w
RMSD bond length	0.010
RMSD bond angle	1.325
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.870	1.392
High resolution limit [Å]	1.340	1.344
Rmerge	0.065	0.438
Rmeas	0.073	0.491
Rpim	0.030	0.216
Number of reflections	36367	3598
<I/σ(I)>	16.27	2.82
Completeness [%]	99.9
Redundancy	5.4
CC(1/2)	0.993	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Ammonium Sulfate , 0.1M Sodium, Acetate pH4.6, 25% PEG4000