6YD5

SaFtsZ-UCM151 (comp. 18)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97895
Spacegroup name	C 1 2 1
Unit cell lengths	72.404, 50.182, 88.600
Unit cell angles	90.00, 111.49, 90.00

Refinement procedure

Resolution	41.220 - 1.550
R-factor	0.1865
Rwork	0.184
R-free	0.23360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3voa
RMSD bond length	0.006
RMSD bond angle	0.896
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.220	1.600
High resolution limit [Å]	1.550	1.550
Rmerge	0.060	0.250
Rmeas	0.090	0.360
Rpim	0.060	0.250
Number of reflections	41526	4196
<I/σ(I)>	5.76	1.78
Completeness [%]	96.2
Redundancy	3.2
CC(1/2)	0.980	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% PEG5K MME, 10% EthGly, 0.2 M Li2SO4, 0.1 M Tris pH 8.5