6YD1
SaFtsZ-DFMBA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2018-12-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97901 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.334, 51.139, 87.823 |
| Unit cell angles | 90.00, 110.44, 90.00 |
Refinement procedure
| Resolution | 41.150 - 1.700 |
| R-factor | 0.1742 |
| Rwork | 0.172 |
| R-free | 0.21870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3voa |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.477 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.150 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.020 | 0.340 |
| Rmeas | 0.030 | 0.480 |
| Rpim | 0.020 | 0.340 |
| Number of reflections | 32199 | 3208 |
| <I/σ(I)> | 13.79 | 1.89 |
| Completeness [%] | 98.2 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.990 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25% PEG5K MME, 10% EthGly, 0.2 M Li2SO4, 0.1 M Tris pH 8.5 |
