6YCY

Plasmodium falciparum Myosin A full-length, post-rigor state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	77.36
Detector technology	PIXEL
Collection date	2019-07-28
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.906019
Spacegroup name	P 21 21 21
Unit cell lengths	89.672, 114.685, 169.461
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.700 - 2.550
R-factor	0.197
Rwork	0.195
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6i7d
RMSD bond length	0.010
RMSD bond angle	1.190
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.700	2.641
High resolution limit [Å]	2.550	2.550
Number of reflections	57507	5563
<I/σ(I)>	10.57
Completeness [%]	99.6
Redundancy	11.2
CC(1/2)	0.998	0.505

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	1.9M Ammonium sulfate, 0.1M Sodium HEPES pH 6.8, 2% PEG400