6YC6

Structure of C. glutamicum GlnK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9184
Spacegroup name	P 43 21 2
Unit cell lengths	82.511, 82.511, 170.587
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.160 - 2.200
R-factor	0.2092
Rwork	0.208
R-free	0.23420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bzq
RMSD bond length	0.004
RMSD bond angle	0.567
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.160	2.279
High resolution limit [Å]	2.200	2.200
Rmerge	0.102	1.750
Number of reflections	30720	2999
<I/σ(I)>	15.34	1.18
Completeness [%]	99.9	100
Redundancy	8.6	8.2
CC(1/2)	0.999	0.458

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	2:0M ammonium sulfate, 5% (v/v) 2-propanol