6YC2
Crystal structure of the light-driven sodium pump KR2 in the pentameric form at room temperature, pH 8.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2019-10-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 135.540, 240.130, 138.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.1643 |
| Rwork | 0.163 |
| R-free | 0.18810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rew |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.119 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.860 | 48.860 | 2.550 |
| High resolution limit [Å] | 2.500 | 12.750 | 2.500 |
| Rmerge | 0.206 | 0.059 | 2.481 |
| Rmeas | 0.213 | 0.062 | 2.558 |
| Rpim | 0.052 | 0.019 | 0.616 |
| Total number of observations | 1274833 | 8610 | 73586 |
| Number of reflections | 78232 | 660 | 4441 |
| <I/σ(I)> | 10.6 | 35.9 | 1.1 |
| Completeness [%] | 100.0 | 98.4 | 100 |
| Redundancy | 16.3 | 13 | 16.6 |
| CC(1/2) | 0.998 | 0.997 | 0.770 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8 | 293 | 1.2 NaMal pH 8.0 |
