6YBI
RT structure of HEW Lysozyme obtained at 1.12 A resolution from crystal grown in a Mylar microchip.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 293.5 |
| Detector technology | PIXEL |
| Collection date | 2017-03-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.170, 79.170, 37.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.580 - 1.120 |
| R-factor | 0.1342 |
| Rwork | 0.133 |
| R-free | 0.15250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iee |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.198 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.580 | 1.160 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Number of reflections | 39456 | 1152 |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 84.6 | |
| Redundancy | 20.14 | |
| CC(1/2) | 0.995 | 0.551 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 4.5 | 293.5 | 5% w/v NaCl, 50 mM AcNa pH 4.5 |
