6YBF
RT structure of HEW Lysozyme obtained at 1.13 A resolution from crystal grown in a Kapton microchip.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 293.5 |
Detector technology | PIXEL |
Collection date | 2017-03-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.110, 79.110, 38.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.550 - 1.130 |
R-factor | 0.1452 |
Rwork | 0.144 |
R-free | 0.16010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iee |
RMSD bond length | 0.012 |
RMSD bond angle | 1.173 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.550 | 1.170 |
High resolution limit [Å] | 1.130 | 1.130 |
Number of reflections | 44967 | 3710 |
<I/σ(I)> | 12.7 | 0.7 |
Completeness [%] | 98.1 | 82.08 |
Redundancy | 11.56 | |
CC(1/2) | 0.997 | 0.700 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | COUNTER-DIFFUSION | 4.5 | 293.5 | 5% w/v NaCl, 50 mM AcNa pH 4.5 |