6YBC
RNASE 3/1 version2 phosphate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792568 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 43.160, 61.740, 51.521 |
| Unit cell angles | 90.00, 106.46, 90.00 |
Refinement procedure
| Resolution | 38.580 - 1.490 |
| R-factor | 0.1514 |
| Rwork | 0.147 |
| R-free | 0.19310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2k11 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.972 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.580 | 1.620 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Number of reflections | 21301 | 1062 |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 99.4 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.996 | 0.828 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM sodium citrate, pH 6.5 and 1 M ammonium phosphate |
