6YAS
HYDROXYNITRILE LYASE FROM HEVEA BRASILIENSIS, ROOM TEMPERATURE STRUCTURE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1997-06 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 47.649, 109.054, 128.654 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.200 |
R-factor | 0.159 * |
Rwork | 0.159 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yas |
RMSD bond length | 0.011 |
RMSD bond angle | 2.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | |
High resolution limit [Å] | 2.120 | 2.200 * |
Rmerge | 0.050 * | 0.132 * |
Number of reflections | 18312 | |
<I/σ(I)> | 11.7 | |
Completeness [%] | 85.1 | 88.3 * |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 0.1 M NATRIUM HEPES BUFFER PH=7.5 2 % PEG 400, 2.0 M AMMONIUM SULFATE, 20 DEGREES. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG400 | 2 (%) | |
2 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
3 | 1 | reservoir | sodium HEPES | 0.1 (M) |