Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-15 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.173, 78.180, 112.664 |
| Unit cell angles | 90.00, 100.68, 90.00 |
Refinement procedure
| Resolution | 49.500 - 2.298 |
| Rwork | 0.205 |
| R-free | 0.23890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xvc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.630 |
| Data reduction software | XDS (Jan 26, 2018) |
| Data scaling software | XDS (Jan 26, 2018) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.500 | 2.430 |
| High resolution limit [Å] | 2.298 | 2.298 |
| Number of reflections | 59126 | 9359 |
| <I/σ(I)> | 7.8 | 1.4 |
| Completeness [%] | 98.9 | 97.8 |
| Redundancy | 7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 29% PEG 4000, 0.1 M ammonium sulphate, 0.1 M sodium citrate, pH 5.6 |
