6Y98

Crystal Structure of subtype-switched Epithelial Adhesin 9 to 1 A domain (Epa9-CBL2Epa1) from Candida glabrata in complex with beta-lactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97242
Spacegroup name	P 31 2 1
Unit cell lengths	65.289, 65.289, 121.877
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.630 - 2.800
R-factor	0.2062
Rwork	0.204
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cp0
RMSD bond length	0.005
RMSD bond angle	1.074
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.450	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.073	1.400
Rmeas	0.077	1.480
Rpim	0.025	0.475
Number of reflections	15594	1132
<I/σ(I)>	18.4
Completeness [%]	99.5	100
Redundancy	9.6	9.6
CC(1/2)	0.999	0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M Potassium Sodium tartrate, 20 % (w/v) PEG 3350