6Y93

Crystal structure of the DNA-binding domain of the Nucleoid Occlusion Factor (Noc) complexed to the Noc-binding site (NBS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-27
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	134.139, 60.567, 81.053
Unit cell angles	90.00, 116.88, 90.00

Refinement procedure

Resolution	72.300 - 2.230
R-factor	0.2337
Rwork	0.231
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6s6h
RMSD bond length	0.002
RMSD bond angle	1.027
Data reduction software	DIALS (2.1.0)
Data scaling software	STARANISO (v3.311 29-Sep-2019)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.300	72.300	2.660
High resolution limit [Å]	2.230	8.570	2.230
Rmerge	0.108	0.036	0.851
Rmeas	0.112	0.037	0.891
Rpim	0.031	0.010	0.258
Number of reflections	10830	541	542
<I/σ(I)>	9.3
Completeness [%]	38.1	100	4.7
Redundancy	13.1	13	11.4
CC(1/2)	1.000	1.000	0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	NULL