6Y8Z

Structure of Baltic Herring (Clupea Harengus) Phosphoglucomutase 5 (PGM5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.07227
Spacegroup name	P 2 21 21
Unit cell lengths	47.200, 94.210, 146.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.930 - 2.050
R-factor	0.1896
Rwork	0.187
R-free	0.23550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6y8y
RMSD bond length	0.002
RMSD bond angle	0.496
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3546)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.900	2.110
High resolution limit [Å]	2.050	2.050
Rmerge	0.124	1.023
Rmeas	0.140	1.222
Rpim	0.062	0.636
Number of reflections	38168	2184
<I/σ(I)>	7.5	2
Completeness [%]	91.2	69
Redundancy	4.8	4.5
CC(1/2)	0.948	0.429

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.12M calcium acetate, 0.08 M sodium cacodylate pH 6.5, 14.0% (v/v) PEG 8000, 20% (v/v) glycerol