6Y7V
Crystal structure of the KDEL receptor bound to HDEL peptide at pH 6.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-28 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.969 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.620, 37.820, 62.420 |
| Unit cell angles | 90.00, 95.18, 90.00 |
Refinement procedure
| Resolution | 36.160 - 2.241 |
| R-factor | 0.2393 |
| Rwork | 0.237 |
| R-free | 0.27540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6i6h |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.860 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.820 | 2.270 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.098 | 0.760 |
| Rmeas | 0.160 | |
| Rpim | 0.097 | 0.570 |
| Number of reflections | 10570 | 407 |
| <I/σ(I)> | 6.9 | 1.6 |
| Completeness [%] | 97.1 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.988 | 0.980 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6 | 292 | 30% (v/v) PEG 600, 100 mM MES pH 6.0, 100 mM Sodium Nitrate. |
