6Y75

BIL2 domain from T.thermophila BUBL1 locus (C1A-N143A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	76.821, 67.605, 82.445
Unit cell angles	90.00, 114.58, 90.00

Refinement procedure

Resolution	47.570 - 2.300
R-factor	0.256
Rwork	0.254
R-free	0.30180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6tmm
RMSD bond length	0.003
RMSD bond angle	0.943
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.120
Rmeas	0.150
Number of reflections	34430	10795
<I/σ(I)>	7.23
Completeness [%]	99.1	99
Redundancy	3.36	3.41
CC(1/2)	0.990	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	293	Mg formate 0.1M, PEG3350 19%, Tris 50mM, NaCl 250 mM.