6Y6T

Mouse Galactocerebrosidase complexed with galacto-noeurostegine GNS at pH 4.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	H 3 2
Unit cell lengths	250.830, 250.830, 77.897
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.980 - 2.250
R-factor	0.1608
Rwork	0.159
R-free	0.19810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zr5
RMSD bond length	0.007
RMSD bond angle	0.893
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.980	2.290
High resolution limit [Å]	2.250	2.250
Number of reflections	44274	2225
<I/σ(I)>	8.8
Completeness [%]	100.0
Redundancy	10.1
CC(1/2)	0.998	0.524

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M sodium acetate, 0.1 M sodium cacodylate (pH 6.8), and 34% wt/vol polyethylene glycol 8000