6Y6R
Crystal structure of MINDY1 T335D mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97628 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 99.810, 99.810, 164.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 85.210 - 3.320 |
R-factor | 0.2018 |
Rwork | 0.200 |
R-free | 0.24350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jkn |
RMSD bond length | 0.008 |
RMSD bond angle | 1.792 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 85.280 | 85.280 | 3.580 |
High resolution limit [Å] | 3.320 | 8.770 | 3.320 |
Rmerge | 0.063 | 0.025 | 1.182 |
Rmeas | 0.070 | 0.028 | 1.335 |
Rpim | 0.030 | 0.012 | 0.607 |
Total number of observations | 70094 | 3937 | 11564 |
Number of reflections | 12828 | 790 | 2511 |
<I/σ(I)> | 13.2 | 50.3 | 1.2 |
Completeness [%] | 99.4 | 99.4 | 97.4 |
Redundancy | 5.5 | 5 | 4.6 |
CC(1/2) | 0.999 | 0.999 | 0.721 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.1 M HEPES sodium pH 7.5, 0.8 M Potassium sodium tartrate tetrahydrate |