6Y6Q

Structure of Andes virus envelope glycoprotein Gc in postfusion conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9786
Spacegroup name	H 3 2
Unit cell lengths	92.647, 92.647, 318.545
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.803 - 2.700
R-factor	0.2368
Rwork	0.234
R-free	0.28690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ljz
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.14rc3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.803	39.800	2.830
High resolution limit [Å]	2.700	8.950	2.700
Rmerge	0.140	0.073	1.279
Rmeas	0.149	0.077	1.354
Rpim	0.049	0.026	0.438
Total number of observations		3638	18675
Number of reflections	14898	456	1949
<I/σ(I)>	8.2	26.9	1.2
Completeness [%]	99.8	96.9	99.9
Redundancy	9.4	8	9.6
CC(1/2)	0.997	0.996	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	16% (w/v) PEG 4000, 10% (v/v) 2-propanol, 0.2M (NH4)2SO4, 0.1M Hepes pH 7.5