Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2016-08-04 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 |
| Unit cell lengths | 130.430, 130.430, 221.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.770 - 2.450 |
| Rwork | 0.161 |
| R-free | 0.19680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fmg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.534 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.770 | 2.538 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmeas | 0.086 | 0.526 |
| Number of reflections | 147085 | 13659 |
| <I/σ(I)> | 21.58 | 4.69 |
| Completeness [%] | 94.8 | |
| Redundancy | 8.3 | |
| CC(1/2) | 0.998 | 0.929 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | KSCN, PEG 3350 |
