6Y5W

Crystal structure of the envelope glycoprotein complex of Andes virus in a near postfusion conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980110
Spacegroup name	H 3
Unit cell lengths	130.330, 130.330, 129.501
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.620 - 2.550
R-factor	0.218
Rwork	0.216
R-free	0.26090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ljz 5opg
Data reduction software	XDS
Data scaling software	Aimless (0.5.25)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.14rc3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.623	37.620	2.660
High resolution limit [Å]	2.550	8.830	2.550
Rmerge	0.062	0.022	0.775
Rmeas	0.078	0.028	0.974
Rpim	0.046	0.016	0.582
Total number of observations		1544	8692
Number of reflections	25995	607	3241
<I/σ(I)>	11.2	36	1.4
Completeness [%]	97.3	94.5	99.8
Redundancy	2.6	2.5	2.7
CC(1/2)	0.997	0.998	0.490

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	15-16% (w/v) PEG 3350, 125-400 mM K acetate