6Y5O
The crystal structure of glycogen phosphorylase in complex with 20
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION SUPERNOVA |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2018-05-30 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.069, 128.069, 116.025 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.740 - 2.330 |
| R-factor | 0.16688 |
| Rwork | 0.164 |
| R-free | 0.21210 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.329 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.740 | 2.420 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.085 | 0.596 |
| Number of reflections | 38529 | 3446 |
| <I/σ(I)> | 10.7 | 1.7 |
| Completeness [%] | 92.6 | 80.3 |
| Redundancy | 4.6 | 3.1 |
| CC(1/2) | 0.991 | 0.768 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | 10 mM BES buffer, pH 6.7 |
