6Y49
Crystal structure of the paraoxon-modified A.17kappa antibody FAB fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97631 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.437, 67.043, 65.985 |
| Unit cell angles | 90.00, 107.37, 90.00 |
Refinement procedure
| Resolution | 24.305 - 1.650 |
| R-factor | 0.1804 |
| Rwork | 0.178 |
| R-free | 0.21540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.118 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.040 | 1.500 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Number of reflections | 69683 | 1538 |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 95.3 | |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.15M K/Na tartrate 23% PEG 3350 |
