6Y41

Fibrinogen-like globe domain of human ANGPTL2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-15
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 2 21
Unit cell lengths	153.940, 154.860, 172.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.630 - 1.790
R-factor	0.1931
Rwork	0.192
R-free	0.21470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qnv
RMSD bond length	0.006
RMSD bond angle	0.887
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	75.300	75.360	1.820
High resolution limit [Å]	1.790	4.860	1.790
Rmeas	0.140	0.053	2.941
Rpim	0.038	0.014	0.798
Total number of observations	5302074	268115	255516
Number of reflections	384504	20029	19082
<I/σ(I)>	11.3	37.7	0.9
Completeness [%]	100.0	100	100
Redundancy	13.8	13.4	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	Morpheus A12 (0.06M Divalents, 0.1M Buffer System 3 pH 8.5, 50% v/v Precipitant Mix 4) + 25 mM CaCl2.