6Y3X
Crystal structure of the Francisella novicida lysine decarboxylase LdcF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0744 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 165.271, 318.247, 184.022 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.270 - 3.400 |
| R-factor | 0.1904 |
| Rwork | 0.185 |
| R-free | 0.23430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n75 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.644 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_3689) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 146.700 | 3.600 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmeas | 0.087 | 1.709 |
| Number of reflections | 66664 | 10429 |
| <I/σ(I)> | 12.16 | 0.94 |
| Completeness [%] | 99.0 | 96.6 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.998 | 0.449 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Buffer: 50 mM HEPES pH 7, 25 mM NaCl, 0.1 mM PLP, 1 mM DTT Crystallization condition: 25% Ethylene Glycol |
