6Y2R

Escherichia coli R255A RnlA endoribonuclease (single alanine mutant of RnlA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-18
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980114
Spacegroup name	P 21 21 21
Unit cell lengths	64.230, 104.930, 152.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.850 - 3.890
R-factor	0.215
Rwork	0.211
R-free	0.30090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4i8o
RMSD bond length	0.004
RMSD bond angle	0.693
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.850	4.030
High resolution limit [Å]	3.890	3.890
Rmerge		2.311
Number of reflections	9959	977
<I/σ(I)>	4.44
Completeness [%]	99.6	98.29
Redundancy	10.1
CC(1/2)	0.987	0.549

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	Protein Buffer: 20 mM Tris HCl pH 8, 150 mM NaCl, 1 mM tris(2-carboxyethyl) phosphine. Reservoir solution: 100 mM Tris-HCl, pH 8.5, 8 % PEG 8000.