6Y2Q

Escherichia coli RnlA endoribonuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.972422
Spacegroup name	P 21 21 21
Unit cell lengths	64.320, 100.800, 154.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.900 - 2.990
R-factor	0.2021
Rwork	0.200
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4i8o
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.900	3.092
High resolution limit [Å]	2.986	2.986
Number of reflections	20825	1869
<I/σ(I)>	9.36	1.12
Completeness [%]	98.9	90.42
Redundancy	7	6.4
CC(1/2)	0.996	0.509

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein Buffer: 20 mM Tris HCl pH 8, 150 mM NaCl, 1 mM tris(2-carboxyethyl) phosphine. Reservoir solution: 0.1 M MES monohydrate pH 6.5, 12% PEG 20 000.