6Y2P

Escherichia coli RnlA-RnlB Toxin-Antitoxin System.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980073
Spacegroup name	C 1 2 1
Unit cell lengths	243.320, 133.580, 55.640
Unit cell angles	90.00, 95.11, 90.00

Refinement procedure

Resolution	48.780 - 2.640
R-factor	0.199
Rwork	0.197
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4i8o
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MR-Rosetta
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.780	2.730
High resolution limit [Å]	2.640	2.640
Rmerge	0.110	0.817
Number of reflections	51657	4871
<I/σ(I)>	12.1	1.51
Completeness [%]	99.2
Redundancy	6.77

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Protein Buffer: 20 mM Tris HCl pH 8, 150 mM NaCl, 1 mM tris (2-carboxyethyl) phosphine. Reservoir solution: 0.1 M Tris HCl pH 8.5, 25 % (w/v) SOKALAN CP 5.