6Y27
Crystal structure of HLA-B2709 complexed with the nona-peptide mA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-17 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.060, 82.720, 110.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.770 - 1.380 |
R-factor | 0.1599 |
Rwork | 0.158 |
R-free | 0.19410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a83 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.232 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.450 |
High resolution limit [Å] | 1.380 | 1.380 |
Rmerge | 0.080 | |
Rmeas | 2.020 | |
Number of reflections | 95918 | 12759 |
<I/σ(I)> | 11.9 | 0.8 |
Completeness [%] | 99.2 | 97 |
Redundancy | 4.7 | 3.9 |
CC(1/2) | 0.998 | 0.303 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 20% (w/v) PEG8000, 150 mM NaCl and 100 mM Tris-HCl, pH 7.0 |