6Y24

Crystal structure of fourth KH domain of FUBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-28
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9184
Spacegroup name	C 2 2 21
Unit cell lengths	69.996, 89.054, 28.711
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.020 - 1.860
R-factor	0.1961
Rwork	0.194
R-free	0.24280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lij
RMSD bond length	0.016
RMSD bond angle	1.546
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.040	1.900
High resolution limit [Å]	1.860	1.860
Number of reflections	7912	486
<I/σ(I)>	9.4
Completeness [%]	100.0
Redundancy	6.1
CC(1/2)	0.996	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	3.1 M sodium formate