6Y1Y

CheA dimerization domain of Treponema denticola

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979100
Spacegroup name	C 1 2 1
Unit cell lengths	101.636, 28.770, 82.982
Unit cell angles	90.00, 114.75, 90.00

Refinement procedure

Resolution	75.000 - 1.500
R-factor	0.1882
Rwork	0.187
R-free	0.21120
Structure solution method	AB INITIO PHASING
RMSD bond length	0.006
RMSD bond angle	0.931
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMPLE
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.360	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.063	0.290
Rmeas	0.075	0.350
Rpim	0.041	0.193
Number of reflections	35178	3490
<I/σ(I)>	14.7	5.4
Completeness [%]	96.7	99.31
Redundancy	6.9
CC(1/2)	0.990	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Imidazole pH 7.0, 25% PEG 400